Figure 7

Intrathecal administration of selective antibody anti-bestrophin-1 or anti-anoctamin-1 reverses established allodynia and reduces protein expression. Time-course of the antiallodynic effect of antibodies against bestrophin-1 (Best-1 ab, 2 μg/6 h × 30 h; a) or anoctamin-1 (Ano-1 ab, 2 μg/6 h x 30 h; d) in rats subjected to L5/L6 spinal nerve ligation. Paw withdrawal threshold was assessed every 6 h starting 12 days after spinal nerve ligation. Data are presented as the mean ± SEM for six animals. *Significantly different from the vehicle group (p < 0.05), as determined by two-way ANOVA followed by the Bonferroni test. Western blot analysis of bestrophin-1 (Best-1, b, c) or anoctamin-1 (Ano-1, e, f) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from neuropathic rats with repeated intrathecal administration of antibodies against bestrophin-1 (Best-1 ab) or anoctamin-1 (Ano-1 ab). Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Insets in b–f show a representative blot obtained with bestrophin-1, anoctamin-1 and β-actin primary antibodies. *Significantly different from the vehicle (V) group (p < 0.05), as determined by the Student t test.