Figure 6

Postnatal increase in the EGFP-positive fiber density in the corneal epithelium of TRPM8 ^EGFPf/+ mice. a Representative images of axons containing EGFP-ir in the basal epithelium of cornea in P2 and adult TRPM8 ^EGFPf/+ mice. b EGFP-positive fiber densities in the corneal epithelium of P2 and adult TRPM8 ^EGFPf/+ mice (n = 7 and 5 mice, respectively). The EGFP-positive fiber densities in the dura of P2 and adult TRPM8 ^EGFPf/+ mice are also plotted (same data as in 5a). **p < 0.01, ***p < 0.001, two-way ANOVA with post hoc Bonferroni test. ^### p < 0.001, compared with the P2 dura group. c Percentage change of EGFP-positive axon density from P2 to adulthood in the cornea and dura of TRPM8 ^EGFPf/+ mice (same mice as in b). The percentage change is calculated as (adult-density − P2-density)/P2-density × 100. ***p < 0.001, two-tailed t-test.