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Figure 7 | Molecular Pain

Figure 7

From: Function and postnatal changes of dural afferent fibers expressing TRPM8 channels

Figure 7

Dural application of TRPM8 agonist (-)-menthol inhibits meningeal irritation-induced ongoing nocifensive behavior in adult mice. a Time spent on forepaw wiping and hindpaw scratching around the scalp and periorbital area (within trigeminal V1 dermatome) in 20 min bins in response to dural application of vehicle or IM in adult male mice (n = 12 and 9, respectively). Naïve mice (n = 6) were habituated to the test room and recording cage as mice in other groups but were not subjected to anesthesia exposure, surgery or drug application. b Total duration of nocifensive behavior during the 120 min recording period in mice that received dural application of vehicle or IM (same mice as in a, ***p < 0.001, two-tailed t-test). c Dural application of (-)-menthol (2.8 mM in 20 µl) reduces the duration of vehicle- and IM-induced nocifensive behavior (n = 6 mice in each group; p < 0.001, two-way ANOVA overall effect, **p < 0.01, ***p < 0.001, post hoc Bonferroni test between individual groups). Co-application of menthol and TRPM8 antagonist AMTB (2.8 mM in 20 µl) reverses the effect of menthol (n = 3 mice; **p < 0.01, ***p < 0.001). AMTB does not alter the duration of IM-induced nocifensive behavior (p = 0.72, between IM and IM+ AMTB groups, n = 6 and 3 mice, respectively).

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