GIRK1 and -2-LIs in control DRG neurons. A–A3 GIRK1 is strongly expressed in the perinuclear region (A, A3), and IB4-LI in the non-peptidergic DRG neurons (A1). Hoechst-LI (A2) is a nuclear marker, and open
arrowheads indicate neurons with co-existence (here and below). B–B3 GIRK1-LI is seen throughout the cytoplasm in medium-sized and large neurons (B, B3), NF200-LI is a marker for large myelinated Aβ fiber neurons (B1, B3). C GIRK1-LI is extensively expressed in DRGs, with varying intensities. D–F Double-staining shows co-existence of GIRK1 with Y1R (D), SST1 (E) and SST2A (F). Arrows point to membrane-association of GIRK1 with the respective GPCR. G–J GIRK2-LI is found in cell bodies, and also fibers (boxed area, solid arrowheads) in a DRG (G). GIRK2-LI is associated with the cell membrane in both small (H) and large neurons (G, J), and a strong cytoplasmic staining is found in small neurons (G, I). K–M Double-staining shows that GIRK2 co-exists with SST1 (L), but not with Y1R (K) or SST2A (M). Scale bars indicate 100 μm (C, G), 40 μm (A–A3, B–B3, E, H–J, L, M), 20 μm (D, F, K).