GIRK2-LI in L4-5 segments of control spinal cord. A GIRK2-LI in the L4-5 spinal cord at low magnification. (B–B2) Double-staining shows GIRK2-IR neuron distribution in different dorsal horn laminae, from the superficial to the deeper layers. NeuN-LI is used to identify neurons (here and below). (B3) Typical cytoplasmic staining pattern of GIRK2-LI. (C–C2) GIRK2- (C) and CGRP-LIs (C1) partly overlap in the dorsal horn (C2). (D–D2) GIRK2-LI (D) overlaps with PV-LI (D1) in inner lamina II (D2). Scale bars indicate 500 μm (A), 100 μm (B, C–D2), 20 μm (B1, B2), 10 μm (B3).