Protein and mRNA expression of GIRK2 in DRGs and spinal cord 14 days after axotomy. A, B Percentages of GIRK2+ NPs is significantly decreased in the ipsilateral (n = 8) as compared to the contralateral DRGs (n = 8). C Western blot result showing GIRK2 protein levels confirms this effect (n = 4 per group). D GIRK2 mRNA shows a modest signal in the contralateral DRG, and is down-regulated in the ipsilateral DRG after axotomy (upper panel: low magnification; lower panel: high magnification). E GIRK2-LI (within white frame) in the dorsal horn. F Monitoring fluorescence intensity in the dorsal horn shows down-regulation of GIRK2-LI in all animals (n = 3 per group). **P < 0.01. Scale bars indicate 200 μm (D, upper panel; A and E), 100 μm (D, lower panel).