Table 4 Primer sequences used in Real-Time PCR
Sequence name | Primer sequence | Amplicon size (bp) |
|---|---|---|
Speer7-ps1 | F: 5′-CATGCTCTCATGCTCACCGA-3′ | 70 |
R: 5′-TACGCTGTAGGACCAGAACAC-3′ | ||
uc007pbc.1 | F: 5′-CATCTAGACCCGTAACGCCC-3′ | 340 |
R: 5′-TGGTAGGCAAGCATCCACAG-3′ | ||
ENSMUST00000171761 | F: 5’-TCGGAGACTTCTCTTCCGGT -3’ | 108 |
R: 5′-AAGACAATGCAGATGGGGCA-3′ | ||
ENSMUST00000097503 | F: 5′-AGGTCATCCCACTTTGGTACAC-3′ | 77 |
R: 5′-GAGTTTGGTTTGCGGGGTCT-3′ | ||
Cyp2d9 | F: 5′-TGTCTACCCTGCGCAACTTT-3′ | 71 |
F: 5′-GTGATTGGCCTCCTTGGTCA-3′ | ||
Mnx1 | F: 5′-GAACACCAGTTCAAGCTCAACA-3′ | 129 |
R: 5′-GCTGCGTTTCCATTTCATTCG-3′ | ||
Gapdh | F:5′-TGTTCCTACCCCCAATGTG-3′ | 129 |
R:5′-GTGTAGCCCAAGATGCCCT-3′ |