Fig. 2

TH mRNA and protein expression in rats with distinct patterns of injury-triggered disability. Box and whisker plots showing: a relative TH mRNA expression and; b relative TH protein expression as determined by real-time RT-PCR and western blotting, respectively. Data are shown for behavioural controls (white); sham controls (green); Pain alone (yellow); Pain and Disability (blue); and Pain and Transient Disability (pink). Significance with respect to behavioural controls and sham surgery controls are shown **p < 0.01 (one-way ANOVA, post hoc Bonferroni test). c Examples of two different western blots showing fluorescence for TH protein (60 KDa) and beta-actin (43 KDa). In each example gel specific examples of a protein sample from each control and experimental group, are indicated by a different coloured arrow: behavioural controls (white); sham controls (green); Pain alone (yellow); Pain and Disability (blue); and Pain and Transient Disability (pink). (We have shown entire gels in this figure, Pain alone rats in lanes e, f, g, h, I, k; Pain and Disability rats in lanes a, b, j; Pain and Transient Disability rats in lanes c, d, l; sham control rats in lanes m, n, o; and behavioural controls in lanes p, q).