Fig. 3

TH-expression in the vPAG of rats with distinct patterns of injury-triggered disability. Top row a–d shows photomicrographs of TH immunoreactivity in the vPAG under brightfield illumination. a −6.3 mm caudal to bregma; b −6.8 mm caudal to bregma; c, −7.3 mm caudal to bregma; and d −7.8 mm caudal to bregma. The scale bar represent 1.0 mm. Directly below each photomicrograph, box and whisker plots illustrate (1) the mean number (±SEM) of TH-IR cells; (2) the mean intensity (±SEM) of staining in TH-IR neurons and; (3) the mean density (±SEM) of TH-IR fibers, at each of these rostro-caudal levels of the vPAG in five rats from each of the behaviourally characterized groups. Significant differences between behavioural groups is shown *p < 0.05 (2-way ANOVA, Bonferroni test).