The effects of a repeated subcutaneous administration of koumine in sciatic nerve chronic constriction injury rats. Koumine (0.28, 1.4, or 7 mg kg−1 body weight (bw)), gabapentin (40 mg kg−1 bw), or vehicle was administered twice daily for 7 consecutive days, starting on postoperative day 4. The time course of the effect of koumine on the thermal withdrawal latency (TWL, a) and mechanical withdrawal threshold (MWT, b) revealed that repeated subcutaneous (s.c.) injections of koumine dose-dependently reversed hyperalgesia and allodynia induced by sciatic nerve chronic constriction injury (CCI) neuropathy. The data are presented as the mean ± SEM (n = 6 per group) and were analyzed using two-way repeated measures ANOVA. The significant differences between the groups were determined by Bonferroni post hoc test at each time point. ###
P < 0.001 vs. the sham group; *P < 0.05, **P < 0.01, ***P < 0.001 vs. the vehicle control group.