3α-Hydroxysteroid oxidoreductase immunohistochemical staining in the spinal cord of koumine-treated sciatic nerve chronic constriction injury rats. a 3α-Hydroxysteroid oxidoreductase (3α-HSOR) immunohistochemical staining in the ipsilateral dorsal horn of the lumbar spinal cord (SC, L5–L6). Scale bar 100 μm. b Quantification of the 3α-HSOR expression in the SC after chronic constriction injury (CCI) by fluorescence density analysis. A time-dependent increase in 3α-HSOR fluorescence density was observed within the ipsilateral SC dorsal horn after CCI. The data are presented as the means ± SEM from 5 to 7 rats per group and were analyzed using two-way ANOVA followed by Bonferroni post hoc test at each time point. ##
P < 0.01 vs. the sham group; *P < 0.05 vs. the CCI group.