Immunostaining of the nerve cellular distribution of 3α-hydroxysteroid oxidoreductase in the dorsal horn of rat spinal cord. Left (nerve cells): Photomicrograph of the dorsal horn section labeled with anti-neuronal nuclei (NeuN), anti-ionized calcium binding adaptor molecule 1 (Iba1), and anti-glial fibrillary acidic protein (GFAP) antibody (green). Center (3α-HSOR): The same section was labeled with anti-3α-hydroxysteroid oxidoreductase (3α-HSOR) antibody (red). Right (merged): Photomicrograph of the same section labeled with anti-3α-HSOR antibody and either anti-NeuN, anti-Iba1, or anti-GFAP antibody. Scale bar 5 μm.