Fig. 2
From: Pirt contributes to uterine contraction-induced pain in mice
Pirt-expressing nerves innervate the uterus. a Hematoxylin and eosin-stained section of the uterus. Myometrium and endometrium are indicated by white arrow and blue arrow, respectively. b Image of the uterine section under fluorescence microscopy. In Pirt−/− mice, the entire Pirt-coding region was replaced by an EGFPf-IRESrtTA-ACN-targeting construct, which expresses green fluorescent protein. Thus, Pirt-expressing fibers can be identified by green fluorescence. c Superimposed images of a and b. Pirt-expressing fiber is indicated by a white arrow. d–f Higher resolution views of the boxed areas in a–c, respectively. Fluorescence (g) and bright-field (h) images from two-photon microscopy analysis showing Pirt-expressing nerve fibers in the myometrium (white arrow). Scale bar 100 µm