Fig. 1
From: Mitochondrial and bioenergetic dysfunction in trauma-induced painful peripheral neuropathy
The bioenergetic profile of mouse sciatic nerves can be assessed by Seahorse metabolic assay. Sciatic nerves from mice were isolated and cut into small 1Â mm hemi-segments. Tissue from a 3Â mm long segment of nerve was placed into a single well. a Oxygen consumption rate was measured, and the oxygen consumption rate in response to oligomycin, FCCP, and antimycin A with rotenone was determined. This allowed ex vivo measurement of oxygen consumption specific to total cellular respiration, mitochondrial respiration, mitochondrial ATP production, mitochondrial proton leak, mitochondrial maximal respiration, mitochondrial spare respiratory capacity, and non-mitochondrial respiration. b Extracellular acidification rate can also be measured by this method. Ex vivo measurement of the extracellular acidification rate corresponding to total extracellular acidification, glycolysis, glycolytic capacity, glycolytic reserve, and non-glycolytic acidification can be measured following administration of oligomycin and 2-deoxyglucose to the tissue culture medium