Fig. 4

Microinjection of Lenti-Cdh1 into the ACC alleviated established SNI-induced mechanical allodynia in rats. A Schematic of the allodynia test and microinjection experiments. B Diagram of microinjection location in the ACC. Asterisks represented the injection sites. C GFP expression was observed 72 h after intra-ACC microinjections of Lenti-control (a) or Lenti-Cdh1 (b). Scale bar 100 μm. D Intra-ACC microinjections of Lenti-Cdh1 significantly reduced SNI-induced mechanical allodynia, whereas the Lenti-control injection did not (Sham and SNI + Lenti-control, n = 6; SNI + Lenti-Cdh1, n = 15; *p < 0.05 vs. Sham control; ^# p < 0.05 vs. Lenti-control)