Fig. 1

BLT1 mRNA increased in dorsal horn neurons after peripheral nerve injury. a Expression of BLT1 mRNA in the ipsilateral spinal cord following SNI surgery by conventional RT-PCR. Upper gel panels show PCR products from the dorsal horn of the L4–5 spinal cord taken from naïve and 7 days after SNI. Lower histogram shows the quantification of relative BLT1 mRNA levels (mean ± SEM; *, P < 0.05 compared with naïve). b, c Bright-field images of in situ hybridization showing BLT1 mRNA expression in lamina III of the spinal dorsal horn in naïve (b) and 7 days after SNI (c). Solid arrowheads indicate the positive cells. Scale bar 25 µm