Fig. 5

Confocal images at high magnification showing the morphology and NK-1r expression by the lamina I spinoparabrachial neuronal population in IB4-saporin-injected animals compared to the vehicle-injected sham group at the 21 days time point. Both fusiform and multipolar neurons showed an increase in NK-1r immunoreactivity in each cell (d, e), compared to the sham group (a, b). However, the great majority of pyramidal neurons did not display NK-1r immunoreactivity following IB4-saporin injection at the same time point (c, f). NK-1 receptor—NK-1 (in green), retrograde tracer CTb (in red). Scale bar 20 µm