Skip to main content
Fig. 3 | Molecular Pain

Fig. 3

From: Anoctamin-1 Cl− channels in nociception: activation by an N-aroylaminothiazole and capsaicin and inhibition by T16A[inh]-A01

Fig. 3

Mouse nocifensive behaviors are induced by ANO1-activator and attenuated by ANO1-inhibitor. The average number a and time spent in b nocifensive behavior responses (licks, flicks, lifts and total responses) of mice hind paws (n = 6) were significantly greater following subplantar injections of an N-aroylaminothiazole ANO1-activator (E-act, 5 mM, black bar) than vehicle (white bar) injections. The average number c and time spent in d nocifensive responses of mice hind paws (n = 6) to co-injections of 5 mM E-act with 1.3 mM of ANO1-inhibitor (ANO1-inh&E-act, white bar) were significantly fewer than subplantar injections of 5 mM E-act alone (black bar). All nocifensive behaviors were quantified from videos of the 5 min immediately following subplantar injections. Injection regimens occurred on different days and opposite paws of the same mice. Error bars are SE. (***p > 0.001, **p > 0.01, *p > 0.05)

Back to article page

Molecular Pain

ISSN: 1744-8069

Contact us