Fig. 5

Overexpression of Navβ4-WT of DRG neuron increases Nav1.6r resurgent currents whereas Navβ2-WT does not. DRG neurons were transfected with Nav1.6r and Navβ4-WT, Navβ2-WT or fluorescent protein tag (control). Beta subunits were tagged with fluorescent protein to monitor expression. Representative traces were obtained from transfected DRG neurons and peak resurgent currents are highlighted for control (a black), Navβ4-WT (b blue), and Navβ2-WT(c green). d Overexpression of Navβ4-WT significantly increased the percentage of DRG neurons that generated resurgent current compared to control (p < 0.0001, χ² test). The percentage of DRG neurons that generated resurgent currents was not different between Navβ2-WT and control groups. e Compared to control (black circles, n = 36), overexpression of Navβ4-WT (blue squares, n = 24) increased resurgent current amplitude. Navβ2-WT (green triangles, n = 17) did not alter resurgent current amplitude relative to control. Note that resurgent currents were normalized to peak transient currents and plotted as a function of voltage. Summary data are mean ± SEM