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Fig. 3 | Molecular Pain

Fig. 3

From: Contribution of Piezo2 to endothelium-dependent pain

Fig. 3

Role of the vascular endothelium and Piezo2 in peripheral tissue in oxaliplatin-induced neuropathic pain. a Rats that had been treated with a single intravenous injection of oxaliplatin (2 mg/kg) received, 48 h later, octoxynol-9 (0.5 % solution, injected intravenously; gray bar). The mechanical nociceptive thresholds were evaluated, by the Randall-Sellitto paw withdrawal test, 30 min after octoxynol-9 injection. We observed significant attenuation of the mechanical hyperalgesia induced by oxaliplatin in rats treated with octoxynol-9 when compared to a control group (white bar) (t 10 = 6.923; ****p < 0.0001, when both groups are compared, Student’s t test), indicating a role of the endothelium in this model of chemotherapy-induced neuropathic pain (N = 6 paws per group); b rats received intravenous injection of oxaliplatin (2 mg/kg). 24 h later, the combination of 3 sequences of ODN mismatch (MM, white bar) or antisense (AS, black bar) against Piezo2 mRNA (20 μg of each sequence/μl, added to 2 μl of oligofectamine; total volume: 5 μl) was injected on the dorsum of the hind paw. 6 h later, the paws were submitted to 4 mechanical stimuli, 5 min apart from each other. We observed that in the paws treated with local injection of ODN AS, but not of ODN MM, the decrease in the nociceptive threshold subsequent to the mechanical stimuli was significantly attenuated, indicating a role of peripheral Piezo2 channels in oxaliplatin-induced hyperalgesia. (t 10 = 12.38; ****p < 0.0001, when both groups are compared, Student’s t test; N = 6 paws per group)

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