Fig. 3

Immunofluorescence images of skin tissues in DCP-treated mice. Serial sections of post-fixed skin tissues were used (a) showing representative IF images with antibodies to B1R (green) and the neuronal marker PGP9.5 (red), litter level of B1R was detected in the PGP9.5 positive areas, B1R expression alone (green) in keratinocytes or some dermis areas (b). A representative IF image with antibodies to PGP9.5 was used to observe morphology of skin