Fig. 3
Oxaliplatin increases TRPA1 mRNA as seen with in situ hybridization histochemistry in the DRG. Effect of oxaliplatin (6 mg/kg, i.p.) on TRPA1 mRNA expression in rat DRG (L4–6) neurons. a Photomicrographs showing in situ hybridization for TRPA1 mRNA at day 2 after oxaliplatin (6 mg/kg, i.p.). TRPA1 mRNA-expressing small-sized neurons were more frequently observed after oxaliplatin treatment. b Size distribution of TRPA1-expressing neurons in DRGs in 5 % glucose- and oxaliplatin-treated rats. TRPA1 expression was distributed in all sizes of neurons, but most TRPA1 expression was found in small DRG neurons. c Histogram shows the percent of TRPA1-positive neurons relative to the total neurons. TRPA1 mRNA-expressing neurons were more frequently observed at days 1, 2, 4, and 7 after oxaliplatin administration (6 mg/kg, i.p.) compared to 5 % glucose-treated animals. Data are the mean ± SEM. n = 4 each for 5 % glucose and oxaliplatin administration. *P < 0.05, **P < 0.01 versus 5 % glucose