Fig. 2

Activation of pp38 by Anis in DRGs. a Pre-treatment of DRGs with Anis increased the expression of pp38 in a dose-dependent manner [Anis (10 nM)/Con, 1.64 ± 0.13, n = 4; Anis (100 nM)/Con, 3.06 ± 0.20, n = 4). b Intrathecal application of Anis (10 nM) significantly increased the percentage of pp38 positive neurons in DRGs. Con, Anis: labeled DRG cells with the pp38 antibody in control and Anis treated DRG slices. Percentages of pp38 positive neurons were 20.4 ± 3.1 % (Control) and 74.0 ± 11.4 % (Anis). c Double labeled DRG neurons with pp38 and P2X3R antibodies. About 20 % P2X3R positive cells expressed pp38. Arrows cells labeled with both P2X3R and pp38. Calibration bar 50 µm, *P < 0.05 compared with Con