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Fig. 1 | Molecular Pain

Fig. 1

From: Brain natriuretic peptide constitutively downregulates P2X3 receptors by controlling their phosphorylation state and membrane localization

Fig. 1

Effects of BNP siRNA. a Representative immunocytochemical examples of endogenous BNP expression in mouse TG cultures in control (upper panels) and after BNP siRNA (lower panels). Nuclei are visualized with DAPI (blue); scale bar = 20 µm. b Representative current traces show α,β-meATP (10 µM, 2 s)-evoked P2X3 responses in control, after 48 h of siBNP, anantin application (500 nM, 2–3 h), or after a combination of siBNP treatment with application of anantin or BNP (100 ng/ml, 2–3 h). The scheme at the bottom describes aforementioned experimental protocols. Histograms show average P2X3 current density values (n = 33, 33, 40, 32, 33, respectively; *p < 0.05, Kruskal–Wallis test). Note that effects of BNP silencing and anantin application are not additive and statistically indistinguishable; BNP applied after siBNP depresses P2X3 currents to the control level

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Molecular Pain

ISSN: 1744-8069

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