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Fig. 2 | Molecular Pain

Fig. 2

From: Brain natriuretic peptide constitutively downregulates P2X3 receptors by controlling their phosphorylation state and membrane localization

Fig. 2

Time-course of anantin effects on P2X3 receptor currents. a Histograms together with representative P2X3 currents induced by α,β-meATP (10 µM, 2 s) show time-course of anantin (500 nM) effects on P2X3 currents. Anantin significantly increases mean P2X3 current density values after 1 h treatment already (n = 44, 30, 40, 40 for control, 1, 3 and 24 h treatment, respectively; *p < 0.05, Kruskal–Wallis test). b Representative traces obtained using α,β-meATP (10 µM, 2 s) show P2X3 current amplitudes in control, after 3 h anantin treatment (500 nM) and after 3 h anantin application followed by 5 h wash with standard physiological solution. Histograms show average P2X3 current density values in control, anantin and wash out conditions (n = 42, 40, 33, respectively; *p < 0.05, Kruskal–Wallis test). Note that 5 h wash abolishes anantin effects on P2X3 current density. c Superimposed average traces of scaled P2X3 currents (shown as mean; sem is within the trace thickness), in control and after anantin treatment (n = 67, 76, respectively, Mann–Whitney rank sum test). Note no significant change in the current onset and slowing down of the decay after anantin application; τ1, τ2 are the fast and slow components of P2X3 current desensitization dynamics. Histograms show mean values for τ1 and τ2 desensitization constants of P2X3 receptor currents in control and after 500 nM anantin application (n = 67, 76, respectively; *p < 0.05, Mann–Whitney rank sum test)

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