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Fig. 3 | Molecular Pain

Fig. 3

From: Brain natriuretic peptide constitutively downregulates P2X3 receptors by controlling their phosphorylation state and membrane localization

Fig. 3

Anantin-mediated changes of P2X3 currents are not CGRP-dependent. a Histogram shows mean current density values of P2X3 receptors in control and after application of CGRP 8-37 (1 µM, overnight), anantin (500 nM, 3 h), or their combination (n = 44, 40, 50, 43, respectively; *p < 0.05, Kruskal–Wallis test). Pretreatment with CGRP 8-37 has no influence on anantin-mediated enhancement of P2X3 current density values, suggesting that this effect is not mediated via CGRP release. Scheme on the right describes experimental protocol of anantin and CGRP 8-37 coapplication. b Histograms compare mean current density values of P2X3 receptors in control and after application of CGRP (1 µM, 3 h), anantin (500 nM, 2 h) or their combination (n = 42, 40, 34, 33, respectively; *p < 0.05, Kruskal–Wallis test). Adding CGRP to the anantin treatment does not further increase P2X3 currents. Scheme on the right describes experimental protocol of anantin and CGRP co-application

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