Fig. 7

P2X3 serine phosphorylation in raft and non-raft membrane compartments. a Top panel shows representative example of Western immunoblotting with P2X3 pSer and anti-P2X3 receptor antibodies, summarizing the amount of P2X3 receptors in control and after application of anantin (500 nM, 2–3 h), MβCD (10 mM, 30 min), or their combination. Lower panel shows total P2X3 receptor amount for each experimental condition; β-tubulin used as loading control of the total extract. Histograms at the bottom quantify P2X3 pSer (relative optical density values) for each experimental condition (n = 4; *p < 0.05, Kruskal–Wallis test). Note that anantin effect on P2X3 pSer level is not influenced by MβCD. b Top panel shows representative example of Western immunoblotting with P2X3 pSer and P2X3 anti-P2X3 receptor antibodies, summarizing the amount of P2X3 receptors in lipid raft (R) and non-raft (NR) membrane compartments in control and after application of anantin (500 nM, 2–3 h), MβCD (10 mM, 30 min), or their combination. Lower panel shows total P2X3 receptor amount for each experimental condition; β-tubulin used as loading control of the total extract. Histograms at the bottom quantify the ratio between P2X3 pSer and total P2X3 in lipid raft and non-raft membrane fractions for each experimental condition (n = 4; *p < 0.05, Kruskal–Wallis test). Note that anantin reduces P2X3 pSer equally in both raft and non-raft membrane fractions and this effect is not influenced by MβCD