Expression of CREB protein and phospho-CREB protein in spinal cord in rats and the effect of intrathecal PKA inhibitor, H89, treatment. Panel A. Immunoblot bands of CREB and phospho-CREB protein expression. Panel B. Bar graph summarizing the density of the immunoblot CREB bands. (NS, no significance between vehicle treatment vs. capsaicin treatment group with either ACSF- or H89- treatment). Panel C. Bar graph showing the density of the immunoblotting bands of phospho-CREB protein. (*, p < 0.05; the value from vehicle treatment vs. capsaicin treatment; #, p < 0.05, the value of ACSF-treated vs. H89-treated animals after capsaicin injection; NS, no significance between vehicle vs. capsaicin treatment in groups with H89 intrathecal administration; n = 5 in each group). Open bar, vehicle group; hatched bar, capsaicin group.