Figure 3
From: Sensitization of TRPV1 by EP1 and IP reveals peripheral nociceptive mechanism of prostaglandins
EP1 receptor involvement in PGE2 (1.5 min)-induced potentiation of capsaicin-activated currents in mouse DRG neurons. (A) Representative traces of potentiation of capsaicin-activated currents by a specific EP1 agonist, ONO-DI-004 (10 μM, 1.5 min), and reverse of the PGE2 (1.5 min)-induced potentiation by a specific EP1 antagonist, ONO-8713 (1 μM). Vh: -60 mV. (B) Effects of PGE2 (1 μM), ONO-DI-004 (EP1 Agon., 10 μM), PGE2 plus ONO-8713 (EP1 Antg., 1 μM), PGE2 plus U73122 (3 μM), PGE2 plus U73343 (3 μM), phorbol 12-myristate 13-acetate (PMA, 100 nM) or PGE2 plus PKCε-I (200 μM) on capsaicin-activated currents in DRG neurons from wild type (EP1 +/+) mice, and effects of PGE2 on capsaicin-activated currents in DRG neurons from EP1 -/- mice. Currents are normalized as described in Fig. 1. * p < 0.05 vs. Cont., + p < 0.05 vs. U73343. Numbers in parenthesis indicate cells tested. (C) Co-expression of TRPV1 (green) and PKCε (blue) in mouse DRG. Arrowheads indicate neurons positive for TRPV1 but not for PKCε. Arrows indicate neurons positive for both TRPV1 and PKCε (light blue). Bar, 100 μm.