Most muscle afferents that express ASIC3 also express CGRP. Three photomicrographs of the same DRG section showing: fluorogold transported from a muscle injection (top); ASIC3 immunoreactivity (middle); CGRP immunoreactivity (bottom). Double arrowheads in the bottom image indicate cells positive for each label. Arrows indicate cells positive for fluorogold and for ASIC3, but not for CGRP. 83% (168/202) of ASIC3-positive muscle afferents were CGRP-positive (373 total muscle afferents counted). Scale bar, 40 μm.