Figure 2
SNI and SNL modulate Na v s mRNA expression in mouse DRG. Transcription profile: (A) one week after SNI in pooled L4 and L5 DRG. Nav1.1, Nav1.3, Nav1.6, Nav1.7, Nav1.8 and Nav1.9 were downregulated after SNI. Only Nav1.2 remained unchanged. *p < 0.05, ***p < 0.001, Student’s t test. The bar graph represents the SNI/sham ratios.% changes in transcripts and p-values are found in Table 2. (B) One week after SNL in L4 and L5 DRG. In injured L5 DRG (black bars), Nav1.1, Nav1.6, Nav1.7, Nav1.8 and Nav1.9 were significantly lower. Nav1.2 and Nav1.3 were unchanged. In non-injured L4 DRG (white bars), only Nav1.1 was lower but Nav1.2, Nav1.3, Nav1.6, Nav1.7, Nav1.8 and Nav1.9 remained unchanged. *p < 0.05, **p < 0.01, ***p < 0.001, two-way ANOVA and post hoc Bonferroni tests. The bar graph represents the SNL/sham ratios for L4 and L5 DRG.% change and p-values are found in Table 3. (C) One week after SNI in separated L3, L4 and L5 DRG. Nav1.1 was significantly downregulated in L3 and L4. Nav1.2, Nav1.3 and Nav1.6 remained statistically unchanged in every DRG tested. Nav1.7 was only significantly downregulated in L3. Nav1.8 and Nav1.9 were downregulated in all three DRG. *p < 0.05, **p < 0.01, ***p < 0.001, two-way ANOVA and post hoc Bonferroni tests. The bar graph represents the SNI/sham ratios for L3, L4 and L5 DRG.% change and p-values are found in Table 4.