Figure 9

GluA1/3 mediated the potentiated AMPAR current on the Fos-positive layer V neurons in mice with nerve injury. A, Digitized photomicrograph showing one Fos-GFP-positive (Fos+) (1) or one Fos-GFP-negative (Fos-) (2) neuron was whole-cell patched and dual-labeled by intracellular injection of Alex-594. B, Samples and summarized results showing the AMPAR I-O curve recorded on Fos- neurons in mice with sham surgery (Sham-Fos-) and on Fos- (Nerve injury-Fos-) and Fos + (Nerve injury-Fos+) neurons in mice with nerve injury, respectively. C, Samples and averaged results showing that NASPM only inhibited the AMPAR EPSCs on Fos + neurons in mice with nerve injury but not on Fos- neurons in mice either with nerve injury or sham surgery. D, Plotted figure showing the summarized effect of NASPM on Sham-Fos-, Nerve injury-Fos- and Nerve injury-Fos + neurons. Bar equals to 20 μm in A. *, p < 0.05; ***, p < 0.001.