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Figure 1 | Molecular Pain

Figure 1

From: Itch-associated peptides: RNA-Seq and bioinformatic analysis of natriuretic precursor peptide B and gastrin releasing peptide in dorsal root and trigeminal ganglia, and the spinal cord

Figure 1

Evaluation of sequences and cross reaction of GRP/bombesin related neuropeptides. A: Amino acid sequence comparison of GRP, NMB, Tachykinin and amphibian peptide orthologs. The table depicts amino acid sequences obtained from human and amphibian sources [http://www.ncbi.nlm.nih.gov/protein]. There is conservation of sequence across human, mouse and rat species for all peptides other than GRP. Mouse and rat GRP share 100% sequence identity which is: VSTGAGGGTVLAKMYPRGSHWAVGHLM (differences from the human sequence are highlighted in red). B: The competitive binding figure is reproduced with permission from Panula et al. (1982) [27]. It examines the cross-reaction of a C-terminally directed anti-bombesin antiserum with bombesin, ranatensin, substance P and eledoisin (an amphibian peptide similar to substance P). Ranatensin shares C-terminal sequence similarity with Neuromedin B and fully displaces the iodinated bombesin tracer. Substance P is just beginning to displace the tracer at the concentrations used.

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Molecular Pain

ISSN: 1744-8069

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