Figure 2

Disruption of axonal trafficking in the sciatic nerve prevents IL-6-mediated nociceptive plasticity. A) Disruption of microtubules with the injection of colchicine (100 μg) or nocodazole (10 μg) into the popliteal fossa (PF) of mice prevented the development of mechanical hypersensitivity and hyperalgesic priming caused by intraplantar injection of IL-6 (0.1 ng). N = 6 mice per condition. B) Popliteal fossa injection of the cytoplasmic dynein inhibitor ciliobrevin D (10 μg) prevented the development of mechanical hypersensitivity and hyperalgesic priming caused by Intraplantar injection of IL-6 (0.1 ng). N = 6 mice per condition. C) Right popliteal fossa injection of ciliobrevin D or nocodazole (both at 10 μg) fails to disrupt the effect of IL-6 given into the left hindpaw. N = 6 mice per condition D) Popliteal fossa injection of ciliobrevin D (CBD, 10 μg) at the time of intraplantar injection of IL6 prevented the enhancement of BDNF expression in the L4-L6 DRGs of mice 3 hrs following injections. N = 6 mice per condition. * = p < 0.05 and *** = p < 0.001.