Expression profiling of genes modulated by minocycline in a rat model of neuropathic pain

Molecular Pain

Table 1 The influence of minocycline on lymphocyte and monocyte cell markers 7 days after CCI

Cell Types	CDs	NAIVE	V-CCI	MC-CCI
Lymphocytes
T-cells	CD3 gamma	1.00 ± 0.03	0.90 ± 0.02	0.96 ± 0.02
T-cells	CD3 eppsilon	1.00 ± 0.06	0.92 ± 0.02	1.00 ± 0.01
T-cells	CD3 delta	1.00 ± 0.02	1.00 ± 0.01	1.09 ± 0.02
T-cells	CD4	1.00 ± 0.01	1.04 ± 0.01	1.15 ± 0,04
T-cells	CD8	1.00 ± 0.05	0.94 ± 0,01	1.03 ± 0.01
B-cells	CD19	1.00 ± 0.04	1.06 ± 0.02	0.96 ± 0.00
NK -cells	CD335	1.00 ± 0.04	1.06 ± 0.02	0.96 ± 0.00
Monocytes	CD40	1.00 ± 0.02	1.3 ± 0.01^***	1.14 ± 0.02^###

Microarray analysis of gene expression in neuropathic pain and its modulation by repeated intraperitoneal administration of minocycline (30 mg/kg; 16 h and 1 h before sciatic nerve damage and then twice daily for 7 days) in the lumbar spinal cord. The results of the cDNA microarray analysis are presented as the mean ± SEM. The statistical analysis was performed using an ANOVA and Bonferroni’s test. ***p <0.001 indicates a significant difference when compared to the control (naive rats); ^###p<0.001 indicates a significant difference when compared to the V-CCI rats. Naive (N), vehicle (V; water for injection), minocycline (MC).

ISSN: 1744-8069