Figure 3
SNC80, not ARM390, sensitizes TRPV1 in primary sensory neurons. TG neurons from rats were nucleofected with empty vector (pEYFP-N1, A), human (B), rat (C), or mouse (D) DOPr-YFP cDNA (Hu, Rt, Ms, respectively) and treated with SNC80 (1 μM, 30 min), ARM390 (1 μM, 30 min), or vehicle (0.1% DMSO). Real-time calcium responses from individual YFP-positive cells were measured before and after exposure to capsaicin (CAP, 50 nM) and the net change in intracellular calcium accumulation (Δ[Ca2+]i) was determined. Representative traces (A-D) and the mean ± SEM (E) of the difference in pre- and post-CAP response for the number of cells indicated at the bottom of each bar (E). ***, *p > 0.001, 0.05 by one-way ANOVA.