Figure 1
From: Epigenetic regulation of spinal cord gene expression controls Opioid-Induced Hyperalgesia
Epigenetic effects of chronic morphine treatment on spinal cord gene expression. (A) Schematic representation of experimental timeline showing the daily dosing schedule of morphine treatment. (B-C) Epigenetic effects of escalating dose morphine treatment on spinal cord gene expression changes after 7 days of washout with/without co-administration of SAHA 50 mg/kg daily. Concomitant HDAC inhibition leads to significant up-regulation in Bdnf and Pdyn mRNA expression 7 days after cessation of morphine treatment (B). The promoter regions of Pdyn and Bdnf exon-IV genes were more strongly associated with acetylated histone H3: aceH3K9 (C). Error bars: SEM, n = 6-8/group, *p < 0.05, **p < 0.01 and ***p < 0.001 for comparison with controls. #p < 0.05, ##p < 0.01 and ###p < 0.001 for comparison with morphine group. Data were analyzed by two-way ANOVA followed by Bonferroni post-hoc tests.