Figure 5
From: Epigenetic regulation of spinal cord gene expression controls Opioid-Induced Hyperalgesia
Chronic morphine with SAHA treatment promotes histone acetylation dependent increase in BDNF expression in spinal cord tissue. (A-B) Micrographs of representative spinal cord dorsal horn sections (Scale bar = 100 micron) showing increased number of BDNF+ cells in the dorsal horn 7 days after cessation of morphine and SAHA (M + S) treatment. (C) Quantification of BDNF positive cells in 10–15 randomly (2 slices per mouse) selected high-power fields (HPF, 400X) of spinal cord dorsal horn per animal. (D) BDNF and acetylated H3K9 co-staining in the M + S treatment group, demonstrating strong association between both. Error bars: SEM, n = 4/treatment group. Data analyzed by unpaired two tailed student t-tests, **p < 0.01 for differences between treatment conditions.