Figure 6
From: Epigenetic regulation of spinal cord gene expression controls Opioid-Induced Hyperalgesia
Chronic morphine with SAHA treatment increases the number of BDNF+ expressing spinal cord dorsal horn neurons. (A-C) Double staining for BDNF and NeuN markers demonstrates strong association with BDNF in neuronal cells (Scale bar = 20 micron). (B-D) Quantification of double staining for BDNF and NeuN markers reveals increased percentage of neurons expressing BDNF in lumbar spinal cord dorsal horn tissue (lamina I-II and III-VI) following M + S treatment. (E) Double staining for BDNF and GFAP markers shows less abundant BDNF expression by astrocytes. Error bars: SEM, n = 4/ treatment group with the numbers of NeuN positive cells (neurons) and BDNF positive neurons were counted in 10–15 randomly selected (2 slices per mouse) high-power fields (HPF, 400X) of spinal cord dorsal horn per animal. Data analyzed by unpaired two tailed student t-tests, **p < 0.01 and ***p < 0.001 for differences between treatment conditions.