Preference for warmer temperatures in CGRPα sensory neuron-ablated mice is dependent on TRPM8. Temperature discrimination assay in saline- and DTX-treated (A) Cgrpα-DTR+/- and (B) DTR-Trpm8-/- mice. Floor temperature on each side was maintained at 25°C versus 25°C, 25°C versus 30°C, 20°C versus 30°C, or 30°C versus 40°C. Time spent on each side was measured over a 10 min period and expressed as a percentage relative to the boxed temperature. All values are represented as mean ± SEM. t-tests were used to compare time points between pre-saline/DTX and post-saline/DTX-treated mice. *p < 0.05, ***p < 0005.