Figure 1
From: Optogenetic activation of brainstem serotonergic neurons induces persistent pain sensitization
Selective expression of ChR2-EYFP in serotoninergic (5-HT) neurons in the rostral ventromedial medulla (RVM) of TPH2-ChR2 transgenic mice. Representative RVM images in increasing scales with anti-GFP staining for ChR2-EYFP-positive cells (green), anti-TPH2 staining for 5-HT cells (red), and merged images (yellow) from TPH2-ChR2 transgenic mice. The white squares encircle the magnified area in RVM shown in following images. Scale bars =200 μM (A), 100 μM (B), 50 μM (C) and 20 μM (D).