Figure 3

Blockade of paclitaxel-induced LPA production. Level of 18:1-LPA in the spinal dorsal horn at 24 h after the injection of paclitaxel or vehicle was measured by using MALDI-TOF-MS with Phos-tag. (A) CP-99994 (10 nmol), MK-801 (10 nmol), or aCSF was intrathecally injected at 30 min prior to the paclitaxel injection. Data represent means ± SEM from experiments using 3 mice. *p < 0.05, versus aCSF-vehicle-treated mice; ^# p < 0.05, versus aCSF-paclitaxel-treated mice. (B) Wild-type (WT), Lpar1^−/−, and Lpar3^−/− mice were used to evaluate LPA level at 24 h after paclitaxel administration. Data represent means ± SEM from experiments using 3–5 mice. *p < 0.05, versus vehicle-treated WT mice; ^# p < 0.05, versus paclitaxel-treated WT mice.