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Volume 10 Supplement 1

Proceedings of the Seventh Scientific Meeting of The TMJ Association

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Epigenetic regulation of gene expression and cellular differentiation

Epigenome is defined by a collection of various chromatin modifications, which maintains a chromatin environment that is either permissive or inhibitory for gene expression. While association of histone modifications with expressed or silent genes has been established, it remains unclear how changes in chromatin modifications relate to changes in gene expression. We used ChIP-Seq to analyze the genome-wide changes in chromatin modifications during short-term activation of naïve and memory CD4+ T cells by T cell receptor (TCR) signaling. In resting and activated T cells, expressed genes were strongly associated with “active” modifications (e.g. H3K4me1/2/3, H2A.Z) and RNA Polymerase II (Pol II), while silent genes were typically associated with repressive marks. However, we found that about 20-30% of silent genes were poised; they possessed positive modifications and sometimes even Pol II at their promoters. Interestingly, majority of genes induced during T cell activation were poised in resting T cells even before the TCR signaling was initiated. Our data suggest that T cell memory in which immunological memory state is determined epigenetically-by transcriptional memory and poising.

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

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Barski, A., Cuddapah, S., Kartashov, A.V. et al. Epigenetic regulation of gene expression and cellular differentiation. Mol Pain 10 (Suppl 1), O20 (2014).

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