Distribution and cellular localisation of p62 immunoreactivity in the spinal dorsal horn following SNL. (A) Although ubiquitously expressed (see positive cell bodies in the ventral spinal cord), high levels of p62 were present in the superficial layers of the dorsal horn, particularly on the side ipsilateral to injury (A, insert). Spots of p62 immunoreactivity were detected within glial processes as shown by some p62 immunolabelling within GFAP-positive structures (B and C arrowheads). However, high p62 levels were particularly evident in some neuronal cell, as shown by the strong signal within NeuN-positive cell bodies (D and E arrows). Each image is a single focal plane from spinal cord sections of mice undergone SNL; B, C, D, E show ipsilateral side. Scale bars: A 250 μm; B, D 100 μm; C 20 μm; E, 50 μm.