Effects of overexpressing mutant CREB in adult cingulate neurons. a. Transfected ACC area showing GFP tagged mCREB protein expression. Scaled Bar: 10 μm. b. YFP mouse brain transfected with the CREB vector. The pCMV transfected brain showed no neurodegeneration (right). The mCREB transfected area showed significant neurodegeneration (left). c. Hoechst nucleus staining showed nuclear condensation and fragmentation at the mCREB transfected area (Top). Caspase 3 staining showed that apoptosis occurred at the mCREB transfected area (Lower).