Figure 2

A selective group II mGluR antagonist (EGLU) reverses the inhibitory effect of LY354740. A: Whole-cell voltage-clamp recordings of an individual CeLC neuron in a brain slice from an arthritic rat show that LY354740 (10 nM) alone decreases the EPSC amplitude. This synaptic inhibition is largely reversed during co-administration of EGLU (10 μM). Following washout (>15 min), synaptic strength (peak amplitude) returns to baseline. B: EGLU (10 μM, 10–12 min) has no effect on EPSCs when applied alone (P > 0.05, Mann-Whitney U-test, n = 7) but reverses inhibition induced by LY354740. In the presence of EGLU (10 μM) the inhibitory effects of LY354740 (10 nM) are significantly attenuated (P < 0.05, paired t-test, n = 5). Inhibition of EPSC amplitude is expressed as the percentage of predrug controls (mean ± SE). * P < 0.05.