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Figure 3 | Molecular Pain

Figure 3

From: The μ opioid agonist morphine modulates potentiation of capsaicin-evoked TRPV1 responses through a cyclic AMP-dependent protein kinase A pathway

Figure 3

Inhibition of FSK-stimulated cAMP accumulation by morphine (1 μM). cAMP levels in FLAG-MOP/TRPV1 double stable colonies were measured using an enzyme-immunoassay kit either in unstimulated cells (no FSK), or in cells treated with FSK (50 μM) and IBMX (100 μM). Morphine (1 μM) significantly reduced cAMP levels in FSK-stimulated cells (morphine) compared to stimulated cells treated with water (vehicle). Morphine inhibited cAMP production by 67.0 ± 4.6% for FLAG-MOP/TRPV1 colony 13 (dotted bars) and 79.2 ± 7.4% for colony 21 (cross-hatched bars). Data are presented as mean ± SEM from n = 3 samples read in triplicate. ** p < 0.01 compared to vehicle.

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Molecular Pain

ISSN: 1744-8069

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