Figure 3

Schematic representation of a coronal brain section corresponding to bregma -3.14 [16] showing the location of the VPL and image locations. Ten days after CCI, representative images from in situ hybridization for detection of Nav1.3 mRNA are shown for the ipsilateral (B) and contralateral (C) sides. On the ipsilateral side, only very light Nav1.3 signal was detectable. On the contralateral side, punctuate Nav1.3 signal was present in CCI animals. Higher magnification insets are shown for the ipsilateral (B') and contralateral (C') sides. Quantification of the number of Nav1.3-positive cells exhibiting a neuronal morphology from each group is shown in (D). Compared to intact animals, and to the ipsilateral side after CCI, the contralateral VPL exhibited a significantly (*p < 0.05) increased number of Nav1.3-positive profiles after CCI. Nav1.3 signal intensity was very low in intact animals, and on the ipsilateral side after CCI, whereas on the contralateral side signal intensity was significantly increased after CCI.