Effect of ATPA on mIPSCs in SG neurons. (A) Effect of ATPA (3 μM) on mIPSC frequency. From left to right, traces of mIPSCs were shown before, during and after application of ATPA (3 μM) in one wild-type neuron. (B) Cumulative histograms of mIPSC inter-event interval and amplitude before and during the application of ATPA (3 μM). (C) Typical time course of the effect of ATPAon mIPSC frequency in neurons from wild-type and GluR5-/- mice. (D) The facilitatory effect of ATPA on mIPSC frequency was concentration dependent (0. 3 μM, n = 5; 3 μM, n = 10; 10 μM, n = 4) * P < 0.05 and ** P < 0.01. (E) Pooled data of the effect of ATPA on mIPSC amplitude in wide-type and GluR5-/- mice. (F) Inhibitory effect of LY293558 (30 μM) on mIPSCs in one SG neuron from wild-type mouse (n = 5). * P < 0.05.