Figure 5
From: Nerve injury induces robust allodynia and ectopic discharges in Nav1.3 null mutant mice
Neuropathic pain behaviour is unaffected in global and conditional Na v 1.3 KO mice. Robust mechanical allodynia developed in both global (A), nociceptor-specific (B) and postnatal KO (C) of Nav1.3 but not in sham operated WT (white triangle in A). The histogram to the left shows the baseline before injury. Nociceptor-specific deletion of Nav1.3 was confirmed by PCR on genomic DNA (in B) which shows a product for the floxed Nav1.3 allele in both DRGs and brain. However, only DRGs produce the KO allele. Note that non-neuronal cells in DRG will contribute to the floxed Nav1.3 band. In C Co-staining in sections from adult DRG from NFH-Cre/Rosa26 mouse shows that most sensory neurons express Cre in NFH-Cre mouse.